Wilson Poon on Twitter: "@luijsterburglab @PowerDNS_Bert @ummagummab Could it be related to the T7 RNA polymerase promoter minimal sequence requirement? I think T7 RNA polymerase results in a G(for capping)-GGAGA- start for
HiScribe® SP6 RNA Synthesis Kit | NEB
SP6 RNA Polymerase – MEBEP Bio Science
MAXIscript™ T7/T3 Transcription Kit
Addgene: SP6-VEE-OKS-iM
Effects of saturation mutagenesis of the phage SP6 promoter on transcription activity, presented by activity logos | PNAS
pCMVTNT™ and pTNT™ Vectors
Addgene: pGGE009 Sequences
SP6 promoter Sequencing Primer, 18-mer
HiScribe® SP6 RNA Synthesis Kit | NEB
Addgene: SP6-sgRNA-scaffold Sequences
Addgene: SP6-hCas9-Ce-mRNA Sequences
Addgene: SP6-VEE-IRES-Puro
Effects of saturation mutagenesis of the phage SP6 promoter on transcription activity, presented by activity logos | PNAS